Social businessSept. 23hearing2003/371/ECthe provisionsand regulationsof the EUeco-labelfortextile products, includingnewstandards prohibitincludingseven kinds ofAPEOsurfactants anddischargeby theiragents orformulationscomposed ofwastewater.Changzhou, JiangsuInspection and Quarantineby HPLCadvanced technologies-hydrophilic interaction chromatographyanditsapplicationinthe field ofinspection and quarantine,successfullydeveloped anew method fordetectingAPEO, providing agreentextilenewtechnologypath.
   With the worldeconomic development,people's environmental awarenessgradually increased,"green" has becomethe target pursued bypeople.For the textileindustry, too, the resultingecologicaltextile testingproject is alsoincreasing year by year, alkyl phenolethoxylates(APEO)detectionis one of them
    Great harmsmallmicro-objects
Alkylphenolethoxylatesisanimportantnon-ionicsurfactants,whichtononylphenolethoxylates(NPEO)themostabundant,accountingfor80%,followedbyoctylphenolethoxylates(OPEO),accountingformorethan15%ofpolyoxyethylenelaurylether(DPEO)andtwononylphenolethoxylates(DNPEO)eachaccountedforabout1%.Polyoxyethylenealkylphenolethers havegood wetting, penetration,emulsification, dispersion, solubilizationand washing, widely used inmany industries,in particularthe most widely usedin the detergentindustry,followed byauxiliaries.Enzyme productsinorder to improvethe applicationof the fiberpenetration,enzyme productsare alsoa certain amount ofAPEO.For the samereason, thedyeing andprinting processesare often used informulationsAPEO.It is not difficultto see, APEOwidely usedin the textileindustry.
 
Alkylphenolethoxylates(APEO)biodegradabilityispeople have beenof concern.Inmany partsoftheUnitedStatesMeasuredoverAPEOremovalandresidues,APEObiodegradationrate is generallyover 90%,upfrom 92.5 to 99.8%.Biodegradationprocess producesOP, NPandNP1EO, NP2EOisrecognized environmentalhormonechemicals thatcanharmthe body's normalhormone secretionof chemical substances,which canlead toreduced spermcount,genitalabnormalities,promptingtheorganism'sreproductivecapacitydecreased.Inview of this,from the 1980s onward shave banne dorre stricted APEO sregulations introduced,and the mostlegally bindingEUregulation2003/53/EC.Theregulations,from17 January 2005onwardsexcept in certaincircumstances, such asa binderforpigment printingand so on,the useof APEOstrict limits,who enter theEUtextile and clothingarerequired to provideAPEOdetectionvalid proofand providestextiles andclothingAPEOsallowedlimitof 0.1%(ie1000mg/kg).
   In-depth studyto explore new ideas
Atpresent,nointernationalpublishingandtextilesAPEOsdetecttherelevantstandard.China's national standard fortextiles APEOs detection method sare:GB/T23322-2009"Determinationoftextilesurfactant-alkylphenolethoxylates."ChinaInspectionandQuarantineAPEOstextilesindustry standardforthe detection ofthree ways,namely: SN / T 1850.1-2006 "textilesalkylphenolsandalkylphenolpolyoxyethyleneethersofcontents Part 1: EfficientliquidChromatography",SN/T1850.2-2006"textilesalkylphenolsandalkylphenolpolyoxyethyleneethersDetermination-Part2:Highperformanceliquidchromatography-massspectrometry,"andSN/T1850.1-2010"textilesalkylphenolsandalkylphenolpolyoxyethyleneethersDeterminationpart3:normal phase high performance liquid chromato graphy and liquid chroma to graphy-tandem mass spectrometry."
   ThesemethodsareusedSoxhletextractionmadetextilesAPEOs,theuseofhigh-performance liquidchromatographyseparation, followed byfluorescencedetector or amass selective detectorforqualitative and quantitative analysis,the main differencebetween thevariousmethodsliesinthedifferentchromatographicteWhereC18columnusingrechniques.versed-phaseliquid chromatography(RPLC)separation techniquemost widely used.APEOssimple conditionRPchromatography, the mobile phaseis typicallywater andmethanolorwater,methanol,Andacetonitrile,OPEOsandNPEOs,OPandgoodNPsarestablebaseline separationusing a fluorescencedetectorwith high sensitivityand accuratequantitative results
   However,due to the differentdegrees of polymerizationOPEOsorNPEOsdifferent polarity, retention time,there are differences,so this methoddoes not providean accuratequalitative results.Asthemobile phaseRPLCusingESIionization sourceexact match, LC-MSorLC-MS/MSmethodforAPsandAPEOscommonly used inqualitative and quantitative analysis, GB / T 23322, SN / T 1850.2-2006andSN / T 1850.3-2010wereexpressed.However,due to the different degree of poly merization APEOs mixture of oligomers containing oxyethy lene group APEOs moree asilyionized long-chain,andshort-chain APEOs ionsuppression occurs,the unknown intensit yand unstable,resulting in the actualte stresults large deviations, and generatea variety ofionizationAPEOsadduct ions,includinghydrogenation,plusammonium,sodiumpluspotassiumionsand processing, resulting invaryingthe proportion ofvarious ionswhichled to the selectionof non-ionicquantitative analysisuncertainty.
    Therefore,laboratoriesusuallynormal phaseliquid chromatography(NPLC)method asasupplementforRPLCforAPEOsqualitativeanalysis.NPLCs pecifie din the standard method sareused hexane,isopropanol and wateras the mobile phase,thedifferent degree of polymerizationAPEOsseparation.Sincealargeproportionofthemobilephaseusinghydrophobicn-hexane,sotheuseoftheHPLCsystemmustbecarriedoutbetweenNPLCandRPLCadequatecleaningwork,spend a lot ofmanpower and resources.
Innovationcomplementarityseizure law
ForAPEOsdetectproblemsthat exist inChangzhou, JiangsuInspection and Quarantine BureauTechnicalServiceCenterbyHPLCadvanced technologies-hydrophilic interaction chromatography(HILIC),tostudytheretentionmechanism,andsuccessfully appliedAPEOsqualitativeanalysis.
  HILICis a way toRPLC, NPLCand ionchromatography(IC)of the newcombination of the threechromatographic techniques,the use ofexactly the samewithRPLCmobile phasefortheanalysis of the mechanismNPLCsame retention,whilethe bondingof differentphasesbutalsotheabilitytoseparatetheIChave.ToachieveHILICapplicationsinthe field ofinspection and quarantine,Laboratory first carried out a large number of theoretical study and experimental science, exploration and research of the separation mechanism. On the basis of theoretical research, laboratory positive for HILIC separation of different degree of polymerization of APEOs compound experiment.
The newly developed method laboratory using HILIC column, acetonitrile and water as the mobile phase, the separation of APEOs mixtures oligomer as single degree of polymerization. The whole analysis process only needs 10 minutes, the mobile phase and RPLC perfectly matched, from the process complicated cleaning HPLC system NPLC qualitative process, separation effect to achieve the same as NPLC, added the confirmation of qualitative analysis to quantitative detection of RPLC. Method is put into use, the accuracy of security detection results. At the same time, shorten testing time, save a lot of manpower and material resources.
The results of this study are inspection and Quarantine Bureau of Jiangsu Industrial Products Testing Center Deputy Director Cao Xizhong attention, he will this method included in the draft prepared in the International Wool Textile Organisation (IWTO) standards -- "Determination of alkylphenols and alkylphenol polyethoxylates in wool" (the detection of APs and APEOs in wool), provide technical guidance and standards of service for the detection of APs and APEOs in global wool
To achieve technological breakthroughs in two aspects of basic theory and practical application, reflects the theory with practice, learn in order to practise, learning to use with a long scientific philosophy, but also reflects the laboratory technical personnel constantly seeking innovation, the pursuit of a breakthrough in the practical work,to work as abasis to promote thetheoretical innovation,and then totheorydrivenwork progressagood creativecycle model.ChangzhouBureau of the relevantresponsible person said,will continue to increaseinvestment in scientific research,scientific researchprojects,based on thescientific researchability,scientific and technological achievementscontinuouslyoutput,doscience and technology review,strong science and TechnologyBureau,the realstrongtechnicalinspection and quarantineimage